منابع مشابه
RNA sequence of potato virus X strain HB.
The genomic RNA of the potato virus X (PVX) strain HB, isolated in Bolivia and able to overcome all known resistance genes, has been cloned and sequenced. The PVXHB RNA sequence is 6432 nucleotides long and contains, similarly to the RNAs of other PVX strains, five open reading frames encoding proteins of M(r)s 165.1K, 24.5K, 12.4K, 7.6K and 25.1K (coat protein), respectively. Multiple amino ac...
متن کاملAnalysis of terminal structures of RNA from potato virus X.
The 5'-end structure of potato virus X RNA was determined following enzymatic methylation in vitro. A single 3H-methyl group was introduced into the 2'-position of the 5'-penultimate residue and the end structure was determined as m7GpppG(m)pAp(Xp)3G. This part of the RNA apparently is involved in binding to ribosomes since it can be partially protected against RNase digestion by wheat germ 40S...
متن کاملNucleotide Sequence of Potato Virus
SUMMARY The complete nucleotide sequence of the genomic RNA of the potyvirus potato virus Y strain N (PVYn) was obtained from cloned cDNAs. This sequence is 9704 nucleotides long and can encode a polyprotein of 3063 amino acids. The positions of the cleavage sites at the N terminus of the capsid and cytoplasmic inclusion proteins have been determined. Other putative protein cleavage sites have ...
متن کاملThe 5'-terminal sequence of potato leafroll virus RNA: evidence of recombination between virus and host RNA.
The discrepancy between published sequences of the 5' non-coding regions of RNA of a Scottish (S) and that of Dutch (D), Australian and Canadian isolates of potato leafroll virus (PLRV) was investigated. Reverse transcription followed by amplification by polymerase chain reaction showed that RNA from three distinct Scottish isolates of PLRV contained molecules with 5' ends like that of the orig...
متن کاملEffects of sequence elements in the potato virus X RNA 5' non-translated aft-leader on its translation enhancing activity
The 5' non-translated aft-leader sequence of potato virus X RNA consists of two regions: the a sequence (41 nucleotides with no G) and the fl sequence (42 nucleotides upstream from AUG). The aft-leader has been shown to enhance strongly the expression of adjacent genes in chimeric mRNAs. This phenomenon has been postulated to be due to the unpaired conformation of the 5'-terminal 30 nucleotides...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1993
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-74-10-2251